Am I Pregnant If My Positive Pregnancy Test Turned Negative The Next Day?

For many women, gathering up the courage to take a home pregnancy test is one of the most stressful experiences ever. The waiting period, particularly for women who want to fall pregnant, can be some of the longest minutes in her life. There is nothing more devastating than to take a test one day and have a positive result only to take a second test the following day and get a negative result. Since home pregnancy tests are 97% accurate, the question about whether or not the individual is in fact pregnant is a valid one.

Chemical Pregnancies and Early Miscarriages

One of the most common reasons for a positive result one day followed by a negative result the following day is due to a chemical pregnancy, which is commonly known as an early miscarriage. According to Baby Centre, early miscarriages are miscarriages that occur within the first trimester of pregnancy and is predicted that as many as 25% of all pregnancies end in a miscarriage. Early miscarriages are an extremely common occurrence and may explain why historically, there has been a tradition of not announcing a pending birth until after the first trimester. The signs of an early miscarriage are simply heavier periods and some women can go through their cycle without a clue that they have miscarried.

Overly Sensitive Pregnancy Tests

Home pregnancy tests work by testing a woman’s Human Chorionic Gonadotropin or hCG levels. This hormone is one of the first to increase during pregnancy. Many women who have been trying to get pregnant for a long period of time have access to high end technology and testing methods which are able to pick up the slightest increase in the level of the hormone. If you are testing shortly after conception, the level of hcg in your system will be very low. Testing at different times of the day or drinking too much liquid before testing can product a negative pregnancy test result when the hCG levels are extremely low.

False Negative Pregnancy Test

Another reason why the second test may have a negative result is that the home pregnancy tests may have been faulty and not worked properly. While this is not typically the case, it can occur on rare occasions. Instead of taking a pregnancy test as soon as possible, it is better to wait until your cycle has run its course. Waiting until a period has been missed is much more accurate than taking a pregnancy test shortly after conception.

  • Tiffyann1994

    I went to the health department yesterday and took a pregnancy test. I did not take and HPTs. When I went in I peed in a dixie cup and waited for 5 minutes before the nurse came back in (with urine in my hand the whole time). When she came back in she took my urine and put it on the pregnancy test. The test appeared to be positive (with a faint positive line) then the positive line disappeared after approximently 4 minutes so the docotrs concluded that I am not pregnant. Are the doctors right? and Why did the test change to negative

    • Tabitha Jordan

      Well, what was the result???

  • fiona

    Hi I missed my periods.I did pregnancy tests on 3 consecutive days which all showed positive.However, 2 days before There was brownish discharge and this morning tge test showed negative.I did not go to the doctors.I’m frustrated .please reply

    • Unfortunately, if you keep testing and it continues to show a negative result, you could have had an early miscarriage. It could also be that you are just testing very early on in the pregnancy and the concentration of hcg in the urine can vary up and down. Depending on the sensitivity of the test, that variation may trigger a positive result or not.

      • Divya Kolapalli

        hi i am trying to concive and i missed my period and i got last two months 12/5/15 and 11/6/15 today 11/7/15 i missed my period and can i take pregnancy test or i have to wait for an other week

        • CC

          Hello my dear I just happened to read your message and thought I’d answer you question? You must be very anxious, one lucky child who will be very well received 🙂 If you can afford it I would do the test and check the results, if it is positive you still must go to Dr to have blood work done, if it shows negative you could try in another week or two depending on when you are to start your cycle. When I was pregnant for the first time I took the test the same week I was suppose to start my period. Good luck.

  • Lou

    Hi can anyone help me please I am nearly a month late on my periods ive tooken a test a few weeks ago and I came back negative now I took one earlier on and it was postive for a while I felt really faint when I found out must of been a shock but then the test changed then to negative I do get a lot of the symptoms people have especially the one where I feel there something heavy starting to press in the bottom of my belly does anyone think I could be pregnant?? And when do you think I should do another test again???

    • It can’t hurt to test again now if your period is month late. If it comes back negative, maybe you need to go and see a doctor to find out what is going on.

  • fingerscrossed

    I am 9 days late took a test yesterday morning came back with 1 bright blue line and then another faint line saying positive. I retook a digital test this morning and it said not pregnant. HELP am i prego or what

    • It could be that you are just very early in your pregnancy and the hcg levels were not high enough to trigger the positive on the digital test. Test again tomorrow with your first morning urine and you should get accurate results.

      • melisa garcia

        Hi.. My last period was November 10-17 2014 and I’m pregnant now I’m wondering when I got pregnant. I have a bf and December 6 I got drunk and had sex with any other guy is it possible it’s his kid?

      • melisa garcia

        I REALLY NEED YOUR HELP!!!!!!!!!!!My due date is August 30th 2015.. I need help because I don’t know when I got pregnant… Unfortunately I cheated on my bf on December 6 2014 I had sex with another guy and I’m really hoping he’s not the father.. My bf and I would have sex basically every day and I’m really hoping the baby is his. What do u think

        • brandy

          Well that would be 8 months from December and you actually hold your baby in for 40 weeks 10 months unless you have her early! Your fine ….Don’t stress you couldn’t know till she is here! Congrats –

        • brandy

          Well that would be 8 months from December and you actually hold your baby in for 40 weeks 10 months unless you have her early! Your fine ….Don’t stress you couldn’t know till she is here! Congrats –

          • melisa garcia

            When do u think I got pregnant? Is it possible for a ultrasound to be wrong

        • Fluffy Bunny

          Or you could just not cheat on your boyfriend. Hope you do the right thing and inform the sorry bastard.

        • joe schmo

          Umm, I think you’re a whore

      • Casey Darling

        Hi, I’m new to the site but was hoping for a little help. I have a beautiful and healthy 4 year old boy. Unfortunately my 2nd pregnancy about 6-9 months ago was eggtopic and I was led down the methatrexate however you spell it path. Now the past week I have had allot of pregnancy symptoms tho I couldn’t be more then just about 3 weeks along if I were. Today is supposed to be the day I am ovulating only according to my phone app and when my last period was and I have had abdominal cramping start last night as well as off an on today especially towards my right side. Iv never had ovulation pain before and tho that seems to be what it is I am terrified of another eggtopic insident.

      • Nae


        • Casey Darling

          Even if you are not ovulating there is always a chance of pregnancy no matter what, the odds are just very very much lower

          • Nae

            What you mean by very very low?

  • nicola

    Hi I have had sex every other day 10 days after my period finished and have stopped 7 days before next period. What are my chances of getting pregnant?

  • Hannah rose

    I have had 3 faint positives in the last few days and I just took a test and it came back negative. I was supposed to go on my period on the 22nd and I have not had a sign of my period. I have an ultrasound scheduled for the 28th of December but I am scared that there will be no baby???

  • Valdi

    I did a pregnancy test this morning and it showed positive. A few minutes later the same test showed negative???? Please explain…..I went from excited and nervous to down 🙁

    • Mahogany Soul

      Same thing happened to me!!! All I can suggest is that you seek a doctor…

    • Jacqueline Lopez

      Did you ever find out what happen!? I took 2 pregnancy test well actually 3 and 2 of them turn positive than the next day starting to look like a negative.. I mean the line is still there but its starting to faint.. I dont know what this means.. I have a docs apoointment on wednesday.. But im really confuse and i dont know what to think…..

    • Sandra Berrospi

      I just took a test and its showed positive and a few seconds later showed neg im so confused … it is late like 10pm idk if that makes a difference????

  • jorichia

    I took two at home pregnancy test. One was faint the other said pregnant 1-2weeks. But when I went to the Er two days later they said I’m not pregnant yet my cycle usually comes on the 15th and it’s the 24th

  • Shelly

    Hi guys I took a pregnancy test it came out positive then 2 days after I got a period like thing, here and there not continuous I went to my dr and told him my symptoms and he practically laughed in my face they drew blood and told me they would call in 3-5 days. I’m very nervous has the same happened to anyone else? Is it normal?

  • Emily

    Ok so Im 2 and a half months late on my period. I took a pregnancy test on March 30th and it was positive then I took one that night and it was negative… Since then I wait 3 days and taking another one and I’ve done that 3 times and it’s still negative BUT still no period.. I’m so confused and worried please help

  • Emily

    Here’s a picture of the test I took the 30th

  • Alicia

    Hi, the last period I had was in February and its now May. I did two pregnancy tests, one in March and one in April. They both came back negative yet I still haven’t had my period. I have lots of signs but if I am pregnant, the first trimester would already be over. Please help me. Does anyone know what’s going on or have any advice?

  • Emma louise

    Hi can anybody help I’m 2 weeks late on my period I am irregular though I can often just miss a period but me and me partner are trying for a baby Iv take a first response early test and it showed a second line with in 3-5 minutes (faint) but I had to do more test to be sure I did a first response (not early testing) if that makes a difference and a clear blue they both said negative? IM so confused why did the first test have 2 lines now I’m getting a negative?

  • Nisha

    Hello guys my name is nisha and i have a guestion… My husband and i been trying to conceive for awhile and finally some yrs later I think we are…. Okay my menstrual suppose to came on june10th But came on the 6th I was jst spotting and the 7and 8 I didnt bleed but the 9th I bleed and the 10th it was gone… So the 11th I spotted again the 13th spotted the 15th spotted also the 17 and 18th spotted that was the last but i been really sick… I took 5 test 3 came back positive then took and it was negative what’s going on… Do anyone have any idea

    • komal

      Hi I’m having situation like I’m irregular in periods n got pcos, now I’m trying to concieve,
      I got positive result on strip with faint line before two days n today I got negative results .how’s it???
      I got so much symptoms of pregnancy … im worried abt my situation I think I should go for an ultrasound or blood test

  • Tanisha

    Hello,I have a question.
    My Primary care doctor took blood from me and found I was pregnant. I started to spot more than usual and went to the ER 3 days later and their saying..NO evidence of pregnancy or miscarriage. Can this be possible? Can the ER test be wrong? HELP I’M SOO CONFUSED

  • Aliza

    Hello I’m Aliza I take pregency test its come positive first after few seconds it’s turn negative…. Can you please help me I need some feed back

    • It could be that you are taking the test VERY early in the pregnancy. You could also be interpreting the spread of the dye across the test as a positive result. The only way to know for sure is to test again in a day or two to see if the line stays.

  • Sandra Broxson

    I took a pregnancy test the night of 9/2 2 and it had a really faint second line. I waited to take another one yesterday morning and it had a little darker but still faint. I took 2 more threw out the day yesterday and they both were negative. Can anyone help me wrap my head around if I’m pregnant or not.

    • It could be that you are testing too early? Is your period late yet? If it is REALLY early in the pregnancy, you will get your best results in the morning when you wake up. The hcg would have had time to concentrate while you are sleeping. Throughout the day, you are drinking things which could dilute the hcg concentration to a point that it wouldn’t register on a pregnancy test.

  • Paula Bickham

    Hi I been feeling sick for a week or two my period didn’t come and my boyfriend got me an pregnancy test they both came back positive and then I started bleeding and the er said it could just be my last period because there was in signs of a miscarriage wen they looked inside I stopped bleeding and took a new test it came back negative and now I’m scared is my baby or what somebody please help
    Email me
    I don’t know how to get back to this

    • If it is an early pregnancy, drinking a lot of liquids could dilute the pregnancy hormone so it didn’t show up on the test. If you are concerned, you should go and see your doctor to find out what is going on.

  • SB

    HELP! I had IUD removed on Aug 17, and my first pregnancy test came back positive on Sept 14th. I did not have a period between the time of removal and the test being positive. For the next two weeks I randomly took pregnancy tests because I had them left over. They would always come back positive. This morning I started bleeding and having cramps. I took a test this evening and it came back negative. Could I have miscarried? Would a test come back negative that quickly if I indeed miscarried? I don’t know what to do. I called my OB earlier when I started bleeding and they said that it could be from intercourse (husband and I had sex last night). She said not to worry unless bleeding or pains got worse. What should I do?

    • It is possible, but the negative test could have been because you drank too much liquid during the day. If the bleeding has stopped, you should take another test with first morning urine to see what the results are.

  • nobien

    help! I had positive pregnancy test at ER the day before my period was due, then the following day I did hpt in the afternoon but it came negative ,I am 3 days late today and i bought two more hpt ,they are all negative, does anybody had same experienced with me and got positive after few more days ?thanks

    • Are you testing with first morning urine or during the day when you have been eating and drinking? It could be that you are diluting your urine so the hcg (pregnancy hormone) is diluted.

  • km

    Hey so my last period only lasted for 1 and a half days ( usually 4-6 days) and was 2 days beforw i should have got it. Ive taken a test as soon as i did it ir was positive then after 30 sec is was negative does this mean im not pregnant. Partner is convinced im not but i donno pl help

    • Test again with your first morning urine. By now, the test line should be pretty apparent and stay instead of disappearing if you are pregnant.

  • Diana

    Join the discussion…i had a pt on Monday and it came out positive and had another on Wednesday but it showed negative and am presently seeing my period and it really hurts please help!

    • 🙁 It sounds like you are having an early miscarriage. If the bleeding gets really heavy or the pain continues, contact your doctor.

      • Kristina

        I had brown discharge on Thursday along with period cramps. The brown stuff stopped the next day. I went to doc Friday my primary care and they have me urine pregnancy test and it had two lines one line was faint but the doctor was able to see it then when I left doctor I brought 4 different hpts and all was negative no second line like the doctor. I literally tested 30 mins after I got the faint positive at the doctor. I don’t understand. What could this mean? Had bad nausea, mood swings and tightening in belly lower along with my lower belly feeling like it was stretching and some cramps. Other symptoms as well. My periods are not normal come on different days. Me and my husband had a positive ovulation LH surge on a test on the first of this month and had sex before taking ovulation test and on the day of test and 4 days straight afterwards. Could I be pregnant with all these negative hpts and one faint positive at doctor and with symptoms?

  • Monica

    I took a hpt and it was positive, and I’m late by a couple weeks. Later that night I went to look at it again it was negative. What does that mean

    • Take the tests with your first morning urine. The pregnancy hormone is most concentrated then. If you have been eating and drinking through the day before you test, that could end up giving you a negative due to the hormone in your urine being diluted.

  • Nicole

    Help please,
    I went to the er yesterday because I thought I had kidney infection.I did not but he told me I am pregnant.I came home and took ept digital again said pregnant.Today was day af should be here didnt come but took another ept neg and 3 dollar store tests all neg.So confused

  • Rebecca H

    Hello. I took a pregnancy test yesterday morning with first pee and it told me yes + (positive). I then went out and purchases a teat that would tell me how far along i am and tested this morning with first pee and it said “not pregnant”. Am i pregnant??? I would have just conceived this cycle. My period was supposed to come yesterday. Thanks!!

    • Ashlee

      Same thing happened to me! Did you ever find out whether it was a false positive ??

      • Rebecca H

        I believe it was a chemical pregnancy. Soon after this post i had excruciating cramping which led to bleeding and clotting. It was not like any period ive had before and it only lasted a day and a half. Took another 2 tests both negative. So yea, either false positive or chemical pregnancy.

        I have hope for next time though! Good luck to you! Xo

        • Ashlee

          Yeah , I think mine was a false positive too ! Such an emotional experience but you have to stay positive- good luck to you too!!

  • paige

    I had spotting for 6 hours on day 26 which I have never done I took a first response gold test in afternoon on day 28 I dipped it into a cup it came back positive then the next morning I peed on the other test I had it came back negative then I went to the minute clinic today (day 30) and it came back negative again….. last month I got my period on day 27 and the month before day 29 one 3 months ago day 33. I’m so confused I’m so tired and so moody and emotional I NEVER get like this I have thick white discharge been having weird dreams. I woke up with a SERIOUS craving for orange juice the other day I NEVER drink orange juice! I have a lot of signs saying I’m pregnant but these negative tests are really messing with me I also found out today I have a “trace” of a uti I never get uti’s. I’m getting blood work tomorrow. I really feel pain in my belly like a babys growing in there but I don’t know I’m such a mess because I really want to be pregnant but I’m scared I’m not 🙁

Last modified: March 19, 2014